Rabbit Anti-HPGD Polyclonal Antibody

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Cat# HPA004919

Size : 100ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

HPA004919

hydroxyprostaglandin dehydrogenase 15-(NAD)

Anti-HPGD Antibody

Polyclonal Antibody against HUMAN HPGD

0.2 mg/ml

info@quimigen.pt

Immunohistochemistry analysis in human urinary bladder and cerebral cortex tissues using HPA004919 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.

Product details

Target protein: hydroxyprostaglandin dehydrogenase 15-(NAD)
Target gene: HPGD
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000031613 (97%)RAT - ENSRNOG00000010610 (95%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000017221
Concentration: 0.2 mg/ml

Applications

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Target information

Protein name: hydroxyprostaglandin dehydrogenase 15-(NAD)
Gene name: HPGD
Alternative gene names: SDR36C1,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

Gibbs DC, Fedirko V, Baron JA, Barry EL, Flanders WD, McCullough ML, Yacoub R, Raavi T, Rutherford RE, Seabrook ME, Bostick RMCancer Prev Res (Phila) , 2020 Sep 11; 14(1):65-76. Epub 2020 Sep 112020 Sep 11
Massoner P, Kugler KG, Unterberger K, Kuner R, Mueller LA, Fälth M, Schäfer G, Seifarth C, Ecker S, Verdorfer I, Graber A, Sültmann H, Klocker HPLoS One , 2013; 8(2):e55207. Epub 2013 Feb 42013