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> Rabbit Anti-DDIT3 Polyclonal Antibody

Rabbit Anti-DDIT3 Polyclonal Antibody

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Cat# HPA068416

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA068416
DNA-damage-inducible transcript 3

Anti-DDIT3 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN DDIT3

0.4 mg/ml
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
Anti-DDIT3 Antibody
info@quimigen.pt

Immunohistochemical staining of human thyroid gland shows moderate to strong nuclear positivity in glandular cells.

Product details

  • Target protein: DNA-damage-inducible transcript 3
  • Target gene: DDIT3
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000025408 (84%)RAT - ENSRNOG00000006789 (88%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A117490
  • Concentration: 0.4 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunohistochemistry (IHC) 

Target information

  • Protein name: DNA-damage-inducible transcript 3
  • Gene name: DDIT3
  • Alternative gene names: CHOP, CHOP10, GADD153, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Porter G, Norris MD, Apte M, Merlot AMNeoplasia , 2025 Jan 15; 60:101115. Epub 2025 Jan 152025 Jan 15
  • Muñoz-Guardiola P, Casas J, Megías-Roda E, Solé S, Perez-Montoyo H, Yeste-Velasco M, Erazo T, Diéguez-Martínez N, Espinosa-Gil S, Muñoz-Pinedo C, Yoldi G, Abad JL, Segura MF, Moran T, Romeo M, Bosch-Barrera J, Oaknin A, Alfón J, Domènech C, Fabriàs G, Velasco G, Lizcano JMAutophagy , 2020 May 25; 17(6):1349-1366. Epub 2020 May 252020 May 25