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> Rabbit Anti-CLOCK Polyclonal Antibody

Rabbit Anti-CLOCK Polyclonal Antibody

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Cat# HPA027565-25ul

Size : 25ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA027565
clock circadian regulator

Anti-CLOCK Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN CLOCK

0.3 mg/ml
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
Anti-CLOCK Antibody
info@quimigen.pt

Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Product details

  • Target protein: clock circadian regulator
  • Target gene: CLOCK
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000029238 (87%)RAT - ENSRNOG00000002175 (78%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000025867
  • Concentration: 0.3 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 

Target information

  • Protein name: clock circadian regulator
  • Gene name: CLOCK
  • Alternative gene names: bHLHe8, KAT13D, KIAA0334, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas