Rabbit Anti-CD2AP Polyclonal Antibody

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Cat# HPA003267

Size : 100ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA003267

CD2-associated protein

Anti-CD2AP Antibody

Polyclonal Antibody against HUMAN CD2AP

0.1 mg/ml

info@quimigen.pt

Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using HPA003267 antibody. Corresponding CD2AP RNA-seq data are presented for the same tissues.

Product details

Target protein: CD2-associated protein
Target gene: CD2AP
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000061665 (82%)RAT - ENSRNOG00000011987 (83%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000009342
Concentration: 0.1 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:50 - 1:200
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Target information

Protein name: CD2-associated protein
Gene name: CD2AP
Alternative gene names: CMS,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

Li G, Lu D, Wang J, Yue S, Tan M, Liu M, Gao XLipids Health Dis , 2022 Sep 19; 21:90. Epub 2022 Sep 192022 Sep 19
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas