Reagents and instruments for immunology, cell biology and molecular biology.
 
> Rabbit Anti-CCNE1 Polyclonal Antibody

Rabbit Anti-CCNE1 Polyclonal Antibody

Print Print
Please log in

Cat# HPA018169

Size : 100ul

Brand : Atlas Antibodies

Contact local distributor :


Phone : +1 850 650 7790

HPA018169
cyclin E1

Anti-CCNE1 Antibody

Polyclonal Antibody against HUMAN CCNE1

0.1 mg/ml
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
Anti-CCNE1 Antibody
info@quimigen.pt

Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.

Product details

  • Target protein: cyclin E1
  • Target gene: CCNE1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000002068 (66%)RAT - ENSRNOG00000014786 (66%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000047259
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:1000 - 1:2500
    • Retrieval method: HIER pH6

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 

Target information

  • Protein name: cyclin E1
  • Gene name: CCNE1
  • Alternative gene names: CCNE, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

  • Deng W, Hao Q, Vadgama J, Wu YJ Cancer Sci Clin Ther , 2021 Mar 18; 5(1):134-1532021 Mar 18
  • Meyer AE, Furumo Q, Stelloh C, Minella AC, Rao SNeoplasia , 2020 Oct 15; 22(11):644-658. Epub 2020 Oct 152020 Oct 15