Rabbit Anti-ARPC2 Polyclonal Antibody

Cat# HPA008352

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA008352
actin related protein 2/3 complex, subunit 2, 34kDa

Anti-ARPC2 Antibody

Polyclonal Antibody against HUMAN ARPC2

0.1 mg/ml
info@quimigen.pt

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Product details

  • Target protein: actin related protein 2/3 complex, subunit 2, 34kDa
  • Target gene: ARPC2
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000006304 (99%)RAT - ENSRNOG00000014289 (99%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: actin related protein 2/3 complex, subunit 2, 34kDa
  • Gene name: ARPC2
  • Alternative gene names: ARC34, p34-Arc, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (8)

  • Domingues N, Catarino S, Cristóvão B, Rodrigues L, Carvalho FA, Sarmento MJ, Zuzarte M, Almeida J, Ribeiro-Rodrigues T, Correia-Rodrigues Â, Fernandes F, Rodrigues-Santos P, Aasen T, Santos NC, Korolchuk VI, Gonçalves T, Milosevic I, Raimundo N, Girão HEMBO J , 2024 Jul 23; 43(17):3627-3649. Epub 2024 Jul 232024 Jul 23

  • Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, Davies JS, Barr VA, Kawai T, Delmonte OM, Bosticardo M, Garofalo M, Carneiro-Sampaio M, Somech R, Gharagozlou M, Parvaneh N, Samelson LE, Fleisher TA, Puel A, Notarangelo LD, Boisson B, Casanova JL, Derfalvi B, Rosenzweig SDNat Commun , 2023 Jun 22; 14:3708. Epub 2023 Jun 222023 Jun 22