Description:
Recombinant monoclonal antibody to alpha-V beta 3 integrin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma EM22703.
Alternative Name(s) of Target: αvβ3; Vitronectin receptor subunit alpha; CD51; ITGB3; Platelet membrane glycoprotein IIIa; CD61; P06756; P05106; avb3
Immunogen: This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas
Specificity: This antibody is specific for the extracellular domain of human recombinant transmembrane-truncated (DTM) αvβ3 integrin, a dimeric cell surface protein that mediates cell attachment. αvβ3 integrin is also involved in tumour growth and angiogenesis.
Application Notes: This antibody has been used to specifically bind αvβ3 in Western blots and in flow cytometry, and to specifically stain αvβ3 integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014; Roth et al, 2013). αvβ3 was found to be strongly expressed in some invasive NSCLC samples, though was not expressed by PC3 prostate cells and human prostate tumours. In FC, the EM22703 signal is increased 3–10 fold in the presence of nanomolar concentrations of the antagonistic αvβ3 inhibitor cilengitide, indicating that EM22703 detects a ligand-induced binding site on αvβ3 (Goodman et al, 2012).
Antibody first published in: Goodman et al. Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumours Biol Open. 2012 Apr 15; 1(4): 329–340. PMID:23213423
Note on publication: Describes the generation and characterisation of this antibody.