POLR3A Antibody - middle region - FITC
Cat# ARP48725_P050-FITC
Size : 100ul
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
POLR3A Antibody - middle region : FITC (ARP48725_P050-FITC)
| Datasheets/Manuals | Printable datasheet for anti-POLR3A (ARP48725_P050-FITC) antibody |
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| Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast |
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| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | FITC: Fluorescein Isothiocyanate |
| Application | WB |
| Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human POLR3A |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 79%; Dog: 86%; Guinea Pig: 86%; Horse: 79%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 86%; Yeast: 85% |
| Peptide Sequence | Synthetic peptide located within the following region: AYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDT |
| Concentration | 0.5 mg/ml |
| Blocking Peptide | For anti-POLR3A (ARP48725_P050-FITC) antibody is Catalog # AAP48725 (Previous Catalog # AAPP28774) |
| Subunit | RPC1 |
| Reference | Hu,P., (2002) Mol. Cell. Biol. 22 (22), 8044-8055 |
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| Gene Symbol | POLR3A |
| Gene Full Name | Polymerase (RNA) III (DNA directed) polypeptide A, 155kDa |
| Alias Symbols | ADDH, C160, HLD7, RPC1, WDRTS, RPC155, hRPC155 |
| NCBI Gene Id | 11128 |
| Protein Name | DNA-directed RNA polymerase III subunit RPC1 |
| Description of Target | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. |
| Uniprot ID | O14802 |
| Protein Accession # | NP_008986 |
| Nucleotide Accession # | NM_007055 |
| Protein Size (# AA) | 1390 |
| Molecular Weight | 156kDa |
| Protein Interactions | SUMO2; UBC; ASB13; NEDD4; POLR3H; VPS18; POLR3B; POLR1D; CRCP; POLR1A; POLR3C; POLR1C; SMARCA5; POLR2H; POLR2E; POLR3D; IKBKG; PCBD1; EWSR1; RPAP2; RPAP3; RPAP1; GPN1; RAE1; POLR3E; PKP2; GTF2B; |
