Pig calprotectin (CALP) ELISA kit
Cat# CSB-EQ013485PI-96T
Size : 96T
Brand : Cusabio
Contact local distributor :
Phone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Calprotectin
Abbreviation
CALP
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 91-102 | ||||||
| 1:200 | Average % | 85 | |||||
| Range % | 81-89 | ||||||
| 1:400 | Average % | 103 | |||||
| Range % | 98-107 | ||||||
| 1:800 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
Recovery
| The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 88-97 | |||||
| EDTA plasma (n=4) | 106 | 102-108 | |||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Pig CALP ELISA Kit was designed for the quantitative measurement of Pig CALP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
Citations
- Spray-dried porcine plasma improves piglets' performance and modulates gut immune-related genes in the first week post-weaning R Beltrán-Debón, J Tarradas, D Torrallardona,Animal Bioscience,2025
- CT Chen, AA van der Eijk, JJC Voermans,Gut Pathogens,2025
- ZW Ng'ang'a, N Tous, M Ballester,Frontiers in Veterinary Science,2025
- T Winogrodzki,/,2023
- T Winogrodzki,Journal of Crohn's & colitis,2023
- R Rebucci,Livestock Science,2022
- Slinger KR,Animal,2019
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