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> OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit

OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit

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Cat# STA-817

Size : 96assays

Brand : Cell Biolabs

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Phone : +1 850 650 7790

AGE Competitive ELISA
  • Detect advanced glycation end product formation as low as 0.5 µg/mL
  • AGE-modified BSA and Reduced BSA included as standards
  • Compatible with cell lysates, plasma, serum, and purified proteins

 

OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit
Catalog Number
STA-817
Size
96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit
Catalog Number
STA-817-5
Size
5 x 96 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit, Trial Size
Catalog Number
STA-817-T
Size
32 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Product Details

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage.

Our OxiSelect™ AGE Competitive ELISA kit is designed for rapid detection and quantitation of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.

Advanced Glycation End Product Formation Pathways. CML = N-epsilon-(carboxymethyl)lysine. GOLD = glyoxal-derived lysine dimer. CEL = N-epsilon-(1-carboxyethyl)lysine. MOLD = methylglyoxal-derived lysine dimer. DOLD = 3-deoxyglucosone-derived lysine dimer. 3-DG = 3-deoxyglucosone.

Recent Product Citations
  1. Hassan, M.A. et al. (2023). The Synergistic Influence of Polyflavonoids from Citrus aurantifolia on Diabetes Treatment and Their Modulation of the PI3K/AKT/FOXO1 Signaling Pathways: Molecular Docking Analyses and In Vivo Investigations. Pharmaceutics. 15(9):2306. doi : 10.3390/pharmaceutics15092306.
  2. Liu, SH. et al. (2023). Attenuation of diabetes-mediated muscle atrophy in rats by fish oil enriched omega-3 polyunsaturated fatty acids supplementation. J Food Drug Anal. 31(3):6. doi: 10.38212/2224-6614.3468.
  3. Yamamoto, T. et al. (2023). Protein components of maple syrup as a potential resource for the development of novel anti‑colorectal cancer drugs. Oncol Rep. 50(4):179. doi: 10.3892/or.2023.8616.
  4. Dewidar, B. et al. (2023). Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases. EBioMedicine. 94:104714. doi: 10.1016/j.ebiom.2023.104714.
  5. Jorge-Galarza, E. et al. (2023). Adipose tissue dysfunction serum markers are associated with high density lipoprotein size and glycation in the early stages of type 2 diabetes. Lipids Health Dis. 22(1):89. doi: 10.1186/s12944-023-01847-7.
  6. Lan, K.C. et al. (2023). Resveratrol Alleviates Advanced Glycation End-Products-Related Renal Dysfunction in D-Galactose-Induced Aging Mice. Metabolites. 13(5):655. doi: 10.3390/metabo13050655.
  7. Tripathi, D. et al. (2023). Oxidative and Glycation Damage to Mitochondrial DNA and Plastid DNA during Plant Development. Antioxidants (Basel). 12(4):891. doi: 10.3390/antiox12040891.
  8. Park, Y.S. et al. (2023). Pyruvate Kinase M2: A New Biomarker for the Early Detection of Diabetes-Induced Nephropathy. Int J Mol Sci. 24(3):2683. doi: 10.3390/ijms24032683.
  9. Pavlovic, D. et al. (2023). The GSTO2 (rs156697) Polymorphism Modifies Diabetic Nephropathy Risk. Medicina (Kaunas). 59(1):164. doi: 10.3390/medicina59010164.
  10. Kundu, A. et al. (2022). Tenovin-1 Ameliorates Renal Fibrosis in High-Fat-Diet-Induced Diabetic Nephropathy via Antioxidant and Anti-Inflammatory Pathways. Antioxidants (Basel). 11(9):1812. doi: 10.3390/antiox11091812.
  11. Wu, H.P. et al. (2022). Decreased Monocyte HLA-DR Expression in Patients with Sepsis and Acute Kidney Injury. Medicina (Kaunas). 58(9):1198. doi: 10.3390/medicina58091198.
  12. Pramanik, S. et al. (2022). The Amelioration of Detrimental Biochemical Anomalies by Supplementing B, C, and E Vitamins in Subjects with Type 2 Diabetes Mellitus May Reduce the Rate of Development of Diabetic Retinopathy. J Diabetes Res. doi: 10.1155/2022/3886710.
  13. Zych, M. et al. (2022). Two Bioactive Compounds, Rosmarinic Acid And Sinapic Acid, Do Not Affect The Depleted Glutathione Level In The Lenses Of Type 2 Diabetic Female Rats. Farmacia. 70(4):607-616. doi: 10.31925/farmacia.2022.4.5.
  14. El-Sayed, N.S. et al. (2022). Potential mechanisms underlying the association between type II diabetes mellitus and cognitive dysfunction in rats: a link between miRNA-21 and Resveratrol's neuroprotective action. Metab Brain Dis. doi: 10.1007/s11011-022-01035-z.
  15. Lim, O. et al. (2022). Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy. Brazilian J. Pharm. Sci. doi: 10.1590/s2175-97902022e19652. 
  16. Sabbatinelli, J. et al. (2022). Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study. Cardiovasc Diabetol. 21(1):95. doi: 10.1186/s12933-022-01535-3.
  17. Tan, H. et al. (2022). Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathways. Mol Med. 28(1):58. doi: 10.1186/s10020-022-00481-w.
  18. Gu, M.J. et al. (2022). Glycolaldehyde, an Advanced Glycation End Products Precursor, Induces Apoptosis via ROS-Mediated Mitochondrial Dysfunction in Renal Mesangial Cells. Antioxidants. 11(5):934. doi: 10.3390/antiox11050934.
  19. Wu, T.H. et al. (2022). Increased serum levels of advanced glycation end products are negatively associated with relative muscle strength in patients with type 2 diabetes mellitus. BMC Endocr Disord. 22(1):118. doi: 10.1186/s12902-022-01035-1.
  20. Wu, H.P. et al. (2022). Increased Production of Interleukin-10 and Tumor Necrosis Factor-Alpha in Stimulated Peripheral Blood Mononuclear Cells after Inhibition of S100A12. Curr Issues Mol Biol. 44(4):1701-1712. doi: 10.3390/cimb44040117.
  21. Borymska, W. et al. (2022). Silymarin from Milk Thistle Fruits Counteracts Selected Pathological Changes in the Lenses of Type 1 Diabetic Rats. Nutrients. 14(7):1450. doi: 10.3390/nu14071450.
  22. Abdou, H.M. et al. (2022). Antidiabetic efficacy of Trifolium alexandrinum extracts hesperetin and quercetin in ameliorating carbohydrate metabolism and activating IR and AMPK signaling in the pancreatic tissues of diabetic rats. Biomed Pharmacother. 149:112838. doi: 10.1016/j.biopha.2022.112838.
  23. Karamalakova, Y. et al. (2022). Pulmonary Protein Oxidation and Oxidative Stress Modulation by Lemna minor L. in Progressive Bleomycin-Induced Idiopathic Pulmonary Fibrosis. Antioxidants (Basel). 11(3):523. doi: 10.3390/antiox11030523.
  24. Tripathi, D. et al. (2022). Glycation damage to organelles and their DNA increases during maize seedling development. Sci Rep. 12(1):2688. doi: 10.1038/s41598-022-06454-7.
  25. Schadler, P. et al. (2022). The Association of Blood Biomarkers and Body Mass Index in Knee Osteoarthritis: A Cross-Sectional Study. Cartilage. 13(1):19476035211069251. doi: 10.1177/19476035211069251.
  26. Krisanits, B.A. et al. (2022). Non-enzymatic glycoxidation linked with nutrition enhances the tumorigenic capacity of prostate cancer epithelia through AGE mediated activation of RAGE in cancer associated fibroblasts. Transl Oncol. 17:101350. doi: 10.1016/j.tranon.2022.101350.
  27. Tsai, P.S. et al. (2021). Advanced glycation end products activated endothelial-to-mesenchymal transition in pancreatic islet endothelial cells and triggered islet fibrosis in diabetic mice. Chem Biol Interact. doi: 10.1016/j.cbi.2021.109562.
  28. Igaki, Y. et al. (2021). Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload. J Mol Cell Cardiol. doi: 10.1016/j.yjmcc.2021.01.002.
  29. Pantner, Y. et al. (2021). DJ-1 attenuates the glycation of mitochondrial complex I and complex III in the post-ischemic heart. Sci Rep. 11(1):19408. doi: 10.1038/s41598-021-98722-1.
  30. Waditee-Sirisattha, R. & Kageyama, H. (2021). Protective effects of mycosporine-like amino acid-containing emulsions on UV-treated mouse ear tissue from the viewpoints of antioxidation and antiglycation. J Photochem Photobiol B. 223:112296. doi: 10.1016/j.jphotobiol.2021.112296.

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