Biological Activity

Oxaliplatin (Eloxatin) is a 1,2-diaminocyclohexane (DACH) carrier ligand-based groups of platinum antitumor agent with IC50 of median 1.3, 6.2 and 1.5 mM for SW480 DLD1, HT29, respectively. Eukaryotic cells respond to the presence of oxaliplatin adducts in DNA by activating signal transduction pathways that result in cell cycle arrest, an increase in DNA repair activity and apoptosis. Oxaliplatin was found to be active against C32 and G361 cell lines with IC50 values of 49.48 and 9.07 μM (1 h exposure), 9.47 and 1.30 μM (4 h exposure), and 0.98 and 0.14 μM (24 h exposure), respectively.

DMSO can inactivate Oxaliplatin's activity

Product Citations

• 

  Dokl Biochem Biophys. 2023 Oct.

  Dichotomic Role of Low-Concentration EGCG in the Oxaliplatin Sensitivity of Colorectal Cancer Cells
  Oxaliplatin purchased from AbMole

Chemical Information

Molecular Weight	397.29
Formula	C8H14N2O4Pt
CAS Number	61825-94-3
Solubility (25°C)	Water 3 mg/mL warmed
Storage	4°C, protect from light, dry, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Matthew D Hall, et al. Cancer Res. Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes