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> ONPG – Galactosidase Assay Kit

ONPG – Galactosidase Assay Kit

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Cat# CA080

Size : 500assays

Brand : Canvax Biotech

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Phone : +1 850 650 7790

ONPG β-Galactosidase Assay Kit – Canvax colorimetric enzyme activity detection

ONPG – Galactosidase Assay Kit

Sensitive colorimetric assay kit for β-galactosidase activity using ONPG substrate.

ONPG β-Galactosidase Assay Kit is a colorimetric assay for determining β-galactosidase activity using o-nitrophenyl-β-D-galactopyranoside (ONPG) as substrate. It produces a yellow compound measurable by spectrophotometry.

The assay allows rapid and accurate quantification of enzyme activity in bacterial and cell culture systems, suitable for routine molecular biology experiments.

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500 assays

Categories: Cell Based Assays, Other Assays & Compounds

Detailed information:

Advantages & Features

  • Fast assay: simple workflow with quick readout.

  • Sensitive detection: colorimetric measurement at 420 nm.

  • Versatile use: compatible with cell lysates and bacterial cultures.

  • Quantitative: reliable measurement of enzyme activity.

  • Validated performance: reproducible results across assays.

Specifications

Includes

– 10 mL ONGP Substrate solution (4mg/ml)
– 0.5 ml β-mercaptoethanol
– 10 mL Buffer Lysis
– 10 mL Buffer Assay (10x)
– 70 mL Buffer Stop Solution
– 100 μl β-galactosidase enzyme (0.4U/μl)

Download documentation

Applications

  • Reporter gene assays.

  • Bacterial gene expression analysis.

  • Enzyme kinetics studies.

  • β-galactosidase activity detection.

Tables & Figures

Quality Control

  • Each lot is validated for substrate specificity, sensitivity, and reproducibility using β-galactosidase standards.

Advice

  • Perform assays at recommended temperature for optimal enzyme activity.

  • Avoid contamination that may cause background color development.

  • Use freshly prepared ONPG solution for best performance.

Storage, Shipping & Guarantee

  • Shipped in: Gel Pack.
  • Storage: 4 ºC.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

Beta-galactosidase assay kit, ONPG β-gal assay, o-nitrophenyl-β-D-galactopyranoside assay, β-gal activity assay, Colorimetric β-galactosidase assay

Also known as:

  • Spanish: Kit de ensayo de β-galactosidasa, Ensayo ONPG, Ensayo colorimétrico de β-galactosidasa
  • French: Kit d’analyse β-galactosidase, Test ONPG, Essai colorimétrique β-galactosidase
  • German: β-Galactosidase-Assaykit, ONPG-Test, Kolorimetrischer β-Galactosidase-Assay
  • Italian: Kit saggio β-galattosidasi, Saggio ONPG, Saggio colorimetrico β-galattosidasi

FAQs

Q1: What is the detection principle of this kit?
The assay measures β-galactosidase activity through ONPG hydrolysis at 420 nm.

Q2: Can this kit be used for bacterial cultures?
Yes, it is compatible with bacterial cultures and cell lysates.

Q3: What type of results does the assay provide?
It delivers quantitative and reproducible colorimetric measurements.