Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322).
Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

The data was published in the journal Cells in 2019. PMID: 31366086

The data was published in the journal PLoS One in 2013. PMID: 24204994

The data was published in the journal PLoS One in 2016.PMID: 27023634

The data was published in the journal PLoS One in 2016.PMID: 27023634

The data was published in the journal Redox Biol in 2017.PMID: 28448946

The data was published in the journal Int J Mol Sci in 2017. PMID: 28926934