IHC-P analysis of human pancreas exocrine glands tissue using GTX28925 NOTCH1 antibody.
Dilution : 1:200

Dot blot analysis of Notch1 N-terminal (row 1) and intra (row 2) peptides using GTX28925 NOTCH1 antibody.
Lane 1 : 200 ng
Lane 2 : 66.67 ng
Lane 3 : 22.22 ng
Lane 4 : 7.41 ng
Lane 5 : 2.47 ng
Dilution : 1:1000

Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot in 293 cells transiently transfected with myc-tagged mouse Notch. Bound antibody was detected with a 1:10,000 dilution of HRP conjugated Goat anti Rabbit IgG, and color development. Lane M: markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.

Western Blot of Rabbit anti-Notch1 antibody (GTX28925). Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17s-estradiol. Lane 3: MCF-7 + 10 μM gamma secretase inhibitor. Load: 35 μg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4ºC. Rabbit secondary antibody at 1:10,000 for 45 min at RT.Predicted/Observed size: 80 kDa for Notch1.

IHC-P analysis of exocrine glands of human pancreas using Rabbit anti-Notch1 antibody (GTX28925) at a dilution of 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.