Western Blot (Cell lysate)
Western blot analysis of NANOG in (A) HeLa and (B) NIH/3T3 cell lysate using NANOG polyclonal antibody (Cat # PAB0375) at 2 ug/mL .
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NANOG.
Immunogen
A synthetic peptide corresponding to amino acids 1-50 of human NANOG.
Host
Rabbit
Reactivity
Chimpanzee, Goat, Human, Monkey, Mouse
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of NANOG in (A) HeLa and (B) NIH/3T3 cell lysate using NANOG polyclonal antibody (Cat # PAB0375) at 2 ug/mL . - Gene Info — NANOG
- Interactomes
- Publication Reference
- Cytoplasmic WEE1 promotes resistance to PD-1 blockade through hyperactivation of the HSP90A/TCL1/AKT signaling axis in NANOGhigh tumors.
Suyeon Kim., Hyo-Jung Lee., Seungho Lee., Jo Eun Chung., Se Jin Oh., Kwon-Ho Song., Eunho Cho., Min Kyu Son., Heeju Kwon., Seung-Jong Kim., Chaeleen Lee., Suhwan Chang., Tae Woo Kim.
Cancer Immunology Research 2025 Mar; 0:0.
Application：Western blot, Mouse, B16 melanoma tumor cells (P0 vs P3 lines).
- Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells.
Woo SR, Lee HJ, Oh SJ, Kim S, Park SH, Lee J, Song KH, Kim TW.
Biochemical and Biophysical Research Communications 2018 Sep; 503(3):1812.
Application：WB-Tr, Human, CaSki, CaSki-NANOG, HEK293, HeLa cells.
- Cytoplasmic WEE1 promotes resistance to PD-1 blockade through hyperactivation of the HSP90A/TCL1/AKT signaling axis in NANOGhigh tumors.