MYH9 ELISA Kit (Dog)
Cat# OKEH03958
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
MYH9 ELISA Kit (Dog) (OKEH03958)
| Datasheets/Manuals | Printable datasheet for MYH9 ELISA Kit (Dog) (OKEH03958) |
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| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology MYH9 ELISA Kit (Dog) (OKEH03958) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYH9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYH9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MYH9 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 10.7 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Dog Myosin-9 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | MYH9 |
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| Gene Full Name | myosin heavy chain 9 |
| Alias Symbols | Myosin heavy chain 9, myosin heavy chain, non-muscle IIa, myosin-9, myosin, heavy polypeptide 9, non-muscle, NMMHC II-a, NMMHC-IIA, non-muscle myosin heavy chain IIa. |
| NCBI Gene Id | 481280 |
| Protein Name | Myosin-9 |
| Description of Target | Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10 (By similarity). |
| Uniprot ID | Q258K2 |
| Protein Accession # | NP_001104237.1 |
| Nucleotide Accession # | NM_001110767.1 |
| Protein Size (# AA) | 1960 |
| Molecular Weight | 1960 kDa |
