Use code ELISA25-262 for 25% off all ELISAs through November.
25% off all ELISA kits
Mouse RANTES ELISA Kit
Mouse RANTES ELISA Kit
RayBio® Mouse RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 200 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 - 30 fold |
| Accession Number | P30882 |
| Gene Id | 20304 |
| Gene Symbols | CCL5, SCYA5 |
| Protein Name / Synonyms | C-C motif chemokine 5 (MuRantes) (SIS-delta) (Small-inducible cytokine A5) (T-cell-specific protein RANTES) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse RANTES qIPCR ELISA Kit
As low as $749.00
RayPlex® Mouse RANTES Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse RANTES ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified RANTES (CCL5), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Sirtuin-1 directly binds and deacetylates hepatic PCSK9 thereby promoting the inhibition of LDL receptor degradation
- Species:
- Mouse
- Sample Type:
- Plasma (ApoE-/- mice)
- Year:
- 2025
- Journal Name:
- Cardiovascular Research
- PubMed ID:
- 40653676
Platelet Bone Morphogenetic Protein-4 Mediates Vascular Inflammation and Neointima Formation after Arterial Injury
- Species:
- Mouse
- Sample Type:
- Serum (BMP4Plt−/−/LDLr−/− mice after wire injury)
- Year:
- 2021
- Journal Name:
- Cells
- PubMed ID:
- 34440796
Respiratory Syncytial Virus Matrix Protein-Chromatin Association Is Key to Transcriptional Inhibition in Infected Cells
- Species:
- Mouse
- Sample Type:
- Serum (RSV-infected BALB/c mice)
- Year:
- 2021
- Journal Name:
- Cells
- PubMed ID:
- 34685766
Collagen IV-conveyed signals can regulate chemokine production and promote liver metastasis
- Species:
- Mouse
- Sample Type:
- Conditioned media (cell culture media)
- Year:
- 2018
FJU-C28 inhibits the endotoxin-induced pro-inflammatory cytokines expression via suppressing JNK, p38 MAPK and NF-κB signaling pathways
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant; Serum
- Year:
- 2021
- Journal Name:
- Pharmacology research & perspectives
- PubMed ID:
- 34669271
“Loss of neutrophil polarization in colon carcinoma liver metastases of mice with an inducible, liver-specific IGF-I deficiency.†Oncotarget vol
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Cell culture media)
- Year:
- 2018
Combined sub-optimal doses of rosuvastatin and bexarotene impair angiotensin II-induced arterial mononuclear cell adhesion through inhibition of Nox5 signaling pathways and increased RXR/PPAR and RXR/PPAR interactions
- Species:
- Mouse
- Sample Type:
- Conditioned Media (HUVEC Cells treated with rosuvastatin and bexarotene)
- Year:
- 2015
- PubMed ID:
- 25602514
Antioxidants & Redox Signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Rosu stimulation with ANG-II, HUVEC Cells)
- Year:
- 2015
Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Kidney lysates)
- Year:
- 2012
- Journal Name:
- American Journal of Physiology: Renal Physiology
- PubMed ID:
- 22160774
Streptococcus pneumoniae stimulates a STING- and IFN regulatory factor 3-dependent type I IFN production in macrophages, which regulates RANTES production in macrophages, cocultured alveolar epithelial cells, and mouse lungs
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Bone Marrow Macs with Pneumolysin)
- Year:
- 2012
- Journal Name:
- Journal of Immunology
- PubMed ID:
- 22156592
