Mouse IgG2a, Human ads Antibody
Cat# OASB01584
Size : 1.0mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Goat Anti-Mouse IgG2a Antibody (OASB01584)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Mouse IgG2a Antibody (OASB01584) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Unconjugated |
| Application | FC, IHC-F, IHC-P, ICC, WB, ELISPOT |
| Additional Information | Description: Pooled antisera from goats hyperimmunized with mouse IgG2a; purified by affinity chromatography on mouse IgG2a covalently linked to agarose; reacts with the heavy chain of mouse IgG2a; cross-adsorbed against mouse IgG1, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse IgG2a |
| Purification | Affinity chromatography on mouse IgG2a covalently linked to agarose. |
| Concentration | 1.0 mg/mL |
| Specificity | IgG2a |
| Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: FLISA: ELISA: FITC and BIOT conjugates R-PE, APC, SPRD, R-PE-TXRD, CY5, R-PE-CY7, and APC-CY7 conjugates TRITC and TXRD conjugates AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 0.1 ug/106 cells 1:100-1:400 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Indirect immunostaining in conjunction with mouse IgG2a monoclonal antibodies, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting |
| Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates aresupplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7(R- PE- CY 7), and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.25 mg in 1.0mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified humanparaproteins. |
