Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended dilutions: Flow cytometry 1:2,000 - 1:10,000 IF microscopy 1:500 - 1:2,500
Reactivity
Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Mouse IgG1 γ1 chain
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized Label: Fluorescein Isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 2.9 moles FITC per mole of Rabbit IgG
Reconstitution Method
Restore with 1.0 ml of deionized water or equivalent.
Concentration
lot specific
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation
FITC
Storage
Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid. For extended storage aliquot contents after restoration and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
