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> Mouse Anti-KDM5B Monoclonal Antibody [Clone:CL1129]

Mouse Anti-KDM5B Monoclonal Antibody [Clone:CL1129]

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Cat# AMAb90860-25ul

Size : 25ul

Brand : Atlas Antibodies

Contact local distributor :


Phone : +1 850 650 7790

AMAb90860
lysine (K)-specific demethylase 5B

Anti-KDM5B Antibody

Monoclonal Antibody against HUMAN KDM5B

1.0 mg/ml
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
Anti-KDM5B Antibody
info@quimigen.pt

Immunohistochemistry analysis in human testis and liver tissues using AMAb90860 antibody. Corresponding KDM5B RNA-seq data are presented for the same tissues.

Product details

  • Target protein: lysine (K)-specific demethylase 5B
  • Target gene: KDM5B
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000042207 (86%)RAT - ENSRNOG00000004433 (91%)
  • Clonality: Monoclonal
  • Clone ID: CL1129
  • Isotype: IgG1
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-04274
  • Concentration: 1.0 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 2-10 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100

Validation

  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) Recombinant expression validation in WB using target protein overexpression. 
  • Immunofluorescence in Cell Lines (ICC-IF) 

Target information

  • Protein name: lysine (K)-specific demethylase 5B
  • Gene name: KDM5B
  • Alternative gene names: CT31, JARID1B, PLU-1, RBBP2H1A, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperature.Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Nataraj K, Schonfeld M, Rodriguez A, Sharma M, Weinman S, Tikhanovich ICell Mol Gastroenterol Hepatol , 2024 Sep 28; 19(1):101414. Epub 2024 Sep 282024 Sep 28
  • Djureinovic D, Hallström BM, Horie M, Mattsson JS, La Fleur L, Fagerberg L, Brunnström H, Lindskog C, Madjar K, Rahnenführer J, Ekman S, Ståhle E, Koyi H, Brandén E, Edlund K, Hengstler JG, Lambe M, Saito A, Botling J, Pontén F, Uhlén M, Micke PJCI Insight , 2016 Jul 7; 1(10):e86837. Epub 2016 Jul 72016 Jul 7

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