mouse Anti-G-Protein, alpha, (Goa) (Biotin) Monoclonal Antibody [Clone:2Q484 (2A)]

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of a-subunits (ao1 and a2) derived from alternative splicing of a single gene. ao1 differs from ao2 within the C-terminal third of the deduced amino acid sequence. Some of the literature suggests that Transducin is the generic name for the heterotrimeric G protein or possibly Gt. Transducin is composed of several subunits. G proteins are further categorized into different classes, including Go and Gi, based on their aa sequence. Goa is a subunit of Go. Goa could be one subgroup of Transducin. However, there is some literature stating that Go, Gi, and Transducin are all separate groups of G proteins.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:3000. Detects a single protein band of 39-42kD in bovine and rat membranes.
Optimal dilutions to be determined by researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.