Mouse Anti-CA12 Monoclonal Antibody [Clone:CL0280]

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Cat# AMAb90639

Size : 100ul

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Phone : +1 850 650 7790

Brand : Atlas Antibodies

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Contact local distributor :

Phone : +1 850 650 7790

AMAb90639

carbonic anhydrase XII

Anti-CA12 Antibody

Monoclonal Antibody against HUMAN CA12

0.7 mg/ml

info@quimigen.pt

Immunohistochemical staining of human kidney shows strong membranous and/or moderate cytoplasmic immunoreactivity in subsets of renal tubules, while glomeruli are negative.

Product details

Target protein: carbonic anhydrase XII
Target gene: CA12
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000032373 (88%)RAT - ENSRNOG00000017766 (88%)
Clonality: Monoclonal
Clone ID: CL0280
Isotype: IgG1
Host: Mouse
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Protein A purified
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: MAB-02614
Concentration: 0.7 mg/ml

Applications

Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 1 µg/ml

Validation

Immunohistochemistry (IHC)
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: carbonic anhydrase XII
Gene name: CA12
Alternative gene names: HsT18816,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

Vergara D, Ravaioli S, Fonzi E, Adamo L, Damato M, Bravaccini S, Pirini F, Gaballo A, Barbano R, Pasculli B, Franck J, Fournier I, Salzet M, Maffia MInt J Mol Sci , 2020 Jan 22; 21(3):715. Epub 2020 Jan 222020 Jan 22
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Human Protein Atlas