Monoclonal Mouse Anti-Human IgM Secondary Antibody (clone IM260 + IM373 + ICO-30, Azide-free,BSA-free, IHC, IF, WB)

Cat# LS-C390418-100

Size : 100ug

Brand : LSBio

Contact local distributor :


Phone : +1 850 650 7790

Overview

Antibody:
Human IgM Mouse anti-Human Monoclonal (Azide-free, BSA-free) (IM260 + IM373 + ICO-30) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free

Specifications

Description
Human IgM antibody LS-C390418 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB.
Target
Human IgM
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 + IgG2b,k Monoclonal
Clone
IM260 + IM373 + ICO-30
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free. Also available Unmodified or Ready-to-Use.
Immunogen
Recombinant heavy chain of human IgM (IM260 IM373 & ICO-30).
Specificity
Human IgM
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

tech@quimigen.pt