MMP13 (NM_002427) Human Over-expression Lysate
Cat# LY400869
Size : 100ug
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MMP13 (NM_002427) Human Over-expression Lysate
SKU
LY400869
Transient overexpression lysate of matrix metallopeptidase 13 (collagenase 3) (MMP13) (as WB positive control)
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC224156 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002427, NP_002418 |
| Synonyms | CLG3; MANDP1; MDST; MMP-13 |
| Predicted MW | 53.82 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002418 |
| Locus ID | 4322 |
| Cytogenetics | 11q22.2 |
| Protein Families | WB |
| Product Manuals |
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| FAQs |
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| SDS |
| Recombinant Protein Resources |
| SKU | Description | Size | ||
|---|---|---|---|---|
| LC400869 | Lyophilized MMP13 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug | | |
| TP324156 | Recombinant protein of Human matrix metallopeptidase 13 (collagenase 3) (MMP13), full length, 20 µg | 20 ug | | |
Citations
MMP-13 stimulates osteoclast differentiation and activation in tumour breast bone metastases
Pivetta, Scapolan, Pecolo et al
Breast Cancer Res. (2011) 13 (5), R105 DOI: 10.1186/bcr3047
Pivetta, Scapolan, Pecolo et al
Breast Cancer Res. (2011) 13 (5), R105 DOI: 10.1186/bcr3047
Cited by 117
AI Summary: OriGene's HuSH 29-mer shRNA constructs against MMP-13 were used to stably silence MMP-13 expression in MDA-MB-231 breast cancer cells, enabling the authors to demonstrate that MMP-13 knockdown reduced osteoclast differentiation and bone destruction in models of breast cancer bone metastasis.
Snippet: They further demonstrated that down-regulation of MMP-13 with antisense oligonucleotides significantly reduced bone destruction. ... was abrogated by stably transfecting MDA-MB-231 with HuSH 29-mer shRNA constructs against MMP-13 (Origene Technologies, Rockville, MD
Showing 1-1 of 1 papers.
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