Immunocytochemistry
Immunocytochemical staining of HEK293 with Methylglyoxal monoclonal antibody, clone 9E7 (Cat # MAB19318). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) Methylglyoxal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Methylglyoxal monoclonal antibody, clone 9E7 (Cat # MAB19318).
- Specifications
Product Description
Mouse monoclonal antibody raised against synthetic Methylglyoxal (MG).
Immunogen
Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Host
Mouse
Reactivity
Human
Form
Liquid
Conjugation
ATTO 488
Purification
Protein G Purified
Isotype
IgG2a
Recommend Usage
ELISA (1:1000)
Flow Cytometry (1:50)
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunofluorescence
Immunocytochemistry
Immunocytochemical staining of HEK293 with Methylglyoxal monoclonal antibody, clone 9E7 (Cat # MAB19318). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) Methylglyoxal Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Methylglyoxal monoclonal antibody, clone 9E7 (Cat # MAB19318).Enzyme-linked Immunoabsorbent Assay
Western Blot