IRAK1 Antibody
Cat# OASG03951
Size : 100ul
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
IRAK1 Antibody (OASG03951)
| Datasheets/Manuals | Printable datasheet for anti-IRAK1 (OASG03951) antibody |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC |
| Additional Information | Cellular Localization: Cytoplasm Nucleus Lipid droplet. Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet. Tissue Specificity: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. |
| Reconstitution and Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Immunogen | Synthesized peptide derived from human IRAK-1 around the non-phosphorylation site of T209. Location: 150-230aa. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Specificity | IRAK-1 Polyclonal Antibody detects endogenous levels of IRAK-1 protein. |
| Application Info | WB: 1:500~2000 IHC: 1:100~300 ELISA: 1:20000 |
| Gene Symbol | IRAK1 |
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| Gene Full Name | interleukin-1 receptor-associated kinase 1 |
| Alias Symbols | IRAK, pelle |
| NCBI Gene Id | 3654; |
| Description of Target | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. |
| Uniprot ID | P51617 |
| Molecular Weight | 77 kDa |
