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> IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

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Cat# I8500-10g

Size : 10g

Brand : US Biological

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I8500 IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
-20°C

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli.

Synonyms:
IPTG; Isopropyl-ß-D-thiogalactopyranoside; Isopropyl-ß-D-thiogalactoside

CAS No:
367-93-1

Molecular Formula:
C9H18O5S

Molecular Weight:
238.31

Purity:
≥99%

Appearance:
White crystalline or powder

Identity (IR):
Conforms to structure

pH (5%):
5.0-7.0

Solubility:
Colorless, clear, complete

Dioxane:
Not Detected

Specific Rotation (C=1,H2O):
-29° to -35°

Melting Point:
110-114°C

Water (KF):
≤1%

Blue-White Assay:
Induces b-galactosidase in a pUC 18-containing strain.

Storage and Stability:
Stable for 12 months after receipt at -20°C.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
US Biological reference:|Bulcha JT, et al. 2019. A Persistence Detector for Metabolic Network Rewiring in an Animal. Cell Rep. 26(2):460-468.e4. doi: 10.1016/j.celrep.2018.12.064|1. Alag, R. et al., (2010) Protein Science 19; 1577–1586. 2. Norgaard, P. & Winther, J.R., Biochem J. 358: 269–274 (2001). 3. Dennis, A.M., et al., Bioconjugate Chem. June 22, epub (2010). 1. Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., 2nd Ed., 4: 22-8.57 (1987). 4. Wehinger S., et al. Biochim Biophys Acta. 2015 Jan 5;1852(5):693-708. doi: 10.1016/j.bbadis.2014.12.021. [Epub ahead of print].