IgM (FITC)

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Cat# I1905-01R-1mg

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1905-01R IgM (FITC)

Clone Type

Polyclonal

Host

goat

Source

rat

Isotype

IgG

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for FLISA, Immunomicroscopy, Flow Cytometry, FACS Analysis as well as other antibody based fluorescent assays. Other applications not tested.

Recommended Dilution:
FLISA: 1:10,000-1:50,000
IF Microscopy: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
495nm

Emission Wavelength:
528nm

Fluorochrome/Protein Ratio:
4.8 moles FITC per mole of Goat IgG

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: rat|Concentration: ~1mg/ml |Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC). |Purity: Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities. |Immunogen: Rat IgM mu chain.|Specificity: Recognizes rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-goat serum, rat IgM and rat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other rat heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Rat IgM mu chain.

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purity

Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.

Specificity

Recognizes rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-goat serum, rat IgM and rat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other rat heavy or light chain isotypes.