Reagents and instruments for immunology, cell biology and molecular biology.
 
> IDH1 ELISA Kit (Pig)

IDH1 ELISA Kit (Pig)

Print Print
Please log in

Cat# OKEH07750

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

IDH1 ELISA Kit (Pig) (OKEH07750)

Review this product on BioCompare.com and enter to win a Amazon Gift Card
IDH1 ELISA Kit (Pig) (OKEH07750)
Datasheets/ManualsPrintable datasheet for IDH1 ELISA Kit (Pig) (OKEH07750)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)114-124% (n = 20)110-118% (n = 20)88-100% (n = 20)101-111% (n = 20)
EDTA plasma(n=5)91-104% (n = 20)109-118% (n = 20)106-118% (n = 20)96-105% (n = 20)
heparin plasma(n=5)91-100% (n = 20)90-99% (n = 20)101-111% (n = 20)89-101% (n = 20)
ELISA Kit PrincipleAviva Systems Biology IDH1 ELISA Kit (Pig) (OKEH07750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IDH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IDH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IDH1 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.7% (n = 20)
Mean Inter-assay CV%: <=5.8% (n = 20)
ELISA Kit Component
ComponentAmount
IDH1 Microplate96 Wells (12 x 8 Well strips)
IDH1 Lyophilized Standard2
100X Biotinylated IDH1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
::Unigene: Ssc.3518
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Pig Isocitrate dehydrogenase [NADP] cytoplasmic
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIDH1
Alias SymbolsCytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase.
Protein NameIsocitrate dehydrogenase [NADP] cytoplasmic
Description of TargetIsocitrate dehydrogenase [NADP] cytoplasmic
Uniprot IDP20304