Images
Immunofluorescence
Application

Immunofluorescence

Immunofluorescence staining of SK-N-BE. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.

Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western Blot (Tissue lysate) analysis of rat liver.

Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot (Cell lysate) analysis of human HEK-293 cell lysate.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against full length recombinant human HYOU1.MAB,monoclonal Ab,monoclonal antibody

    Immunogen

    Recombinant protein corresponding to full length human HYOU1.

    Host

    Mouse

    Reactivity

    Human, Mouse, Rat

    Specificity

    This antibody detects ~170 kDa.

    Form

    Liquid

    Conjugation

    APC

    Purification

    Protein G purification

    Isotype

    IgG2b

    Recommend Usage

    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at 4°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    Application Data with Unconjugated Antibody.
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunofluorescence

    Immunofluorescence staining of SK-N-BE. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRP170 Antibody. (D) Composite.

    Immunocytochemistry

    Western Blot (Tissue lysate)

    Western Blot (Tissue lysate) analysis of rat liver.

    Western Blot (Cell lysate)

    Western Blot (Cell lysate) analysis of human HEK-293 cell lysate.
  • Gene Info — HYOU1

    Entrez GeneID

    10525

    Protein Accession#

    Q9Y4L1

    Gene Name

    HYOU1

    Gene Alias

    DKFZp686N08236, FLJ94899, FLJ97572, Grp170, HSP12A, ORP150

    Gene Description

    hypoxia up-regulated 1

    Omim ID

    601746

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq

    Other Designations

    150 kDa oxygen-regulated protein|glucose-regulated protein 170|oxygen regulated protein (150kD)

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  • Diseases
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