This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/20,000).
Reactivity
Bakers Yeast
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Hexokinase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized IgG fraction. Molar radio: Biotin/IgG = ~5.4
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation
Biotin
Storage
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase. Present with 40800 molecules/cell in log phase SD medium.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
