RayBio® Human ICAM-1 (CD54) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human sICAM-1 ELISA Kit
Human sICAM-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 150 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 150 pg/ml - 20 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 200 fold |
| Accession Number | P05362 |
| Gene Id | 3383 |
| Gene Symbols | ICAM1 |
| Protein Name / Synonyms | Intercellular adhesion molecule 1 (ICAM-1) (Major group rhinovirus receptor) (CD antigen CD54) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human sICAM-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified ICAM-1 (CD54 / ICAM1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
High throughput morphological screening identifies chemically defined media for mesenchymal stromal cells that enhances proliferation and supports maintenance of immunomodulatory function
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (MSCs in chemically defined media)
- Year:
- 2025
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 40055728
Single-cell Sequencing Reveals Neutrophil Extracellular Traps in Association with Endotheliopathy and Immunothrombosis in Hepatitis B Virus-related Acute-on-chronic Liver Failure
- Species:
- Human
- Sample Type:
- Plasma (HBV-related ACLF patients)
- Year:
- 2026
- Journal Name:
- Journal of clinical and translational hepatology
- PubMed ID:
- 42181839
Placenta-derived IL-32β activates neutrophils to promote preeclampsia development
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture medium of HUVECs co-cultured with PMNs)
- Year:
- 2021
- Journal Name:
- Cellular & Molecular Immunology
- PubMed ID:
- 33707686
A novel OSA-related model of intermittent hypoxia in endothelial cells under flow reveals pronounced inflammatory pathway activation
- Species:
- Human
- Sample Type:
- Human Umbilical Vein Endothelial Cells (HUVEC)
- Year:
- 2023
- Journal Name:
- Frontiers in physiology
Is analysis of inflammatory biomarkers and lymphocyte subpopulations useful in prediction of tuberculosis treatment outcomes?
- Species:
- Human
- Sample Type:
- Serum (tuberculosis patients)
- Year:
- 2021
- Journal Name:
- Journal of clinical tuberculosis and other mycobacterial diseases
- PubMed ID:
- 34541339
Lack of a Synergistic Effect on Cardiometabolic and Redox Markers in a Dietary Supplementation with Anthocyanins and Xanthophylls in Postmenopausal Women
- Species:
- Human
- Sample Type:
- Plasma (postmenopausal women, supplementation trial)
- Year:
- 2019
- Journal Name:
- Nutrients
- PubMed ID:
- 31284490
Subacute exposure of PM2.5 induces airway inflammation through inflammatory cell infiltration and cytokine expression in rats
- Species:
- Human
- Sample Type:
- Conditioned media (cell culture media, BEAS-2B )
- Year:
- 2020
Intercellular adhesion molecule-1 expression and serum levels as markers of pre-clinical atherosclerosis in polycystic ovary syndrome
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2019
- Journal Name:
- Journal of ovarian research
FAK and Pyk2 activity promote TNF-α and IL-1β-mediated pro-inflammatory gene expression and vascular inflammation
- Species:
- Human
- Sample Type:
- Cell Lysate (HAoEC cell lysates); Tissue Lysate / Homogenate
- Year:
- 2019
- Journal Name:
- Scientific Reports
- PubMed ID:
- 31110200
A multicenter randomized, double-blind, placebo-controlled pilot study to assess the efficacy and safety of riociguat in systemic sclerosis-associated digital ulcers
- Species:
- Human
- Sample Type:
- Plasma (patients)
- Year:
- 2019
- Journal Name:
- Arthritis Research and Therapy