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Human RANTES ELISA Kit
Human RANTES ELISA Kit
RayBio® Human RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 - 50 fold |
| Accession Number | P13501 |
| Gene Id | 6352 |
| Gene Symbols | CCL5, SCYA5, D17S136E |
| Protein Name / Synonyms | C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68) RANTES(4-68)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human RANTES qIPCR ELISA Kit
As low as $639.00
RayPlex® Human RANTES Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human RANTES ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified RANTES (CCL5), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment
- Species:
- Human
- Sample Type:
- M14 cell
- Year:
- 2023
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
Oncosuppressive miRNAs loaded in lipid nanoparticles potentiate targeted therapies in BRAF-mutant melanoma by inhibiting core escape pathways of resistance
- Species:
- Human
- Sample Type:
- A375 cell, M14 res cell
- Year:
- 2022
- Journal Name:
- Oncogene
Tumor-Derived CCL5 Recruits Cancer-Associated Fibroblasts and Promotes Tumor Cell Proliferation in Esophageal Squamous Cell Carcinoma
- Species:
- Human
- Sample Type:
- HCE-7 cell, ESCC-1F cell, FEF3 cell
- Year:
- 2023
- Journal Name:
- Molecular Cancer Research
Tumor specific cytokine pattern explored in renal cell carcinoma
- Species:
- Human
- Sample Type:
- Serum; Tissue Lysate / Homogenate; Urine
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 42809553
CaSR Antagonist (Calcilytic) NPS 2143 Hinders the Release of Neuroinflammatory IL-6, Soluble ICAM-1, RANTES, and MCP-2 from Aβ-Exposed Human Cortical Astrocytes
- Species:
- Human
- Sample Type:
- Other / Unspecified (NAHAs-conditioned growth media)
- Year:
- 2020
- Journal Name:
- Cells
- PubMed ID:
- 32498476
Tumor Extracellular Vesicles Regulate Macrophage-Driven Metastasis through CCL5
- Species:
- Human
- Sample Type:
- Other / Unspecified (media of transduced BM1 cells)
- Year:
- 2021
- Journal Name:
- Cancers
- PubMed ID:
- 34298673
Collagen IV-conveyed signals can regulate chemokine production and promote liver metastasis
- Species:
- Human
- Sample Type:
- Conditioned media (cell culture media)
- Year:
- 2018
Potential role of IGF-1R in the interaction between orbital fibroblasts and B lymphocytes: an implication for B lymphocyte depletion in the active inflammatory phase of thyroid-associated ophthalmopathy
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatant of co-cultured cells)
- Year:
- 2024
- Journal Name:
- BMC Immunology
- PubMed ID:
- 38734625
Fruitflow attenuates human platelet granule secretion through down-regulating Src/PLCγ2/PKC signaling pathway
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (platelet supernatant)
- Year:
- 2020
Relationships Between a Range of Inflammatory Biomarkers and Subjective Sleep Quality in Chronic Insomnia Patients: A Clinical Study
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Nature and science of sleep
