RayBio® Human PAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human PAI-1 ELISA Kit
Human PAI-1 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 35 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 35 pg/ml - 25 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta1, TGF-beta2, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 200 fold |
| Accession Number | P05121 |
| Gene Id | 5054 |
| Gene Symbols | SERPINE1, PAI1, PLANH1 |
| Protein Name / Synonyms | Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human PAI-1 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Serpin E1 (SERPINE1 / PAI-1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
A hybrid epithelial-mesenchymal transition program enables basal epithelial cells to bypass stress-induced stasis and contributes to a metaplastic breast cancer progenitor state
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from HMECs)
- Year:
- 2024
- Journal Name:
- Breast Cancer Research
- PubMed ID:
- 39696672
Effects of Initial Combinations of Gemigliptin Plus Metformin Compared with Glimepiride Plus Metformin on Gut Microbiota and Glucose Regulation in Obese Patients with Type 2 Diabetes: The INTESTINE Study
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Nutrients
Correlations between biomarkers of senescent cell accumulation at the systemic, tissue and cellular levels in elderly patients
- Species:
- Human
- Sample Type:
- fibroblasts (FB), mesenchymal stem/stromal cells (MSC)
- Year:
- 2023
- Journal Name:
- Experimental Gerontology
Adipocyte-Derived Leptin Promotes PAI-1-Mediated Breast Cancer Metastasis in a STAT3/miR-34a Dependent Manner
- Species:
- Human
- Sample Type:
- Conditioned Media (cell culture media)
- Year:
- 2020
- Journal Name:
- Cancers
Beyond glycaemic control: A crossover, doubleblinded, 24week intervention with liraglutide in type 1 diabetes.
- Species:
- Human
- Sample Type:
- plasma
- Year:
- 2018
Effects of sitagliptin therapy on markers of low-grade inflammation and cell adhesion molecules in patients with type 2 diabetes
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2014
- Journal Name:
- Metabolism, Clinical and Experimental
Inhibition Effect of Physalis angulata Leaf Extract on Viability, Collagen Type I, and Tissue Inhibitor of Metalloproteinase 1 (TIMP-1) but Not Plasminogen Activator Inhibitor-1 (PAI-1) of Keloid Fibroblast Culture
- Species:
- Human
- Sample Type:
- keloid fibroblasts
- Year:
- 2023
- Journal Name:
- Clinical Cosmetic and Investigational Dermatology
A Novel Agent with Histone Deacetylase Inhibitory Activity Attenuates Neointimal Hyperplasia
- Species:
- Human
- Sample Type:
- Conditioned Media (Vascular smooth muscles cells)
- Year:
- 2014
- Journal Name:
- Cardiovascular Drugs and Therapy
