Human Anti-CD52 [Alemtuzumab (Campath-1H)] MonoclonalAntibody

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Cat# Ab00124-10.0-BT

Size : 1mg

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Phone : +1 850 650 7790

Brand : Absolute Antibody

Contact local distributor :

Phone : +1 850 650 7790

Datasheet

Description:

Recombinant monoclonal antibody to CD52. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody Alemtuzumab (Campath-1H).

Clone Name:

Alemtuzumab (Campath-1H)

Published Application(s):

Cytotoxicity Assay, IHC-P, in vivo, WB, ELISA, FC, IHC-Fr

Published Species Reactivity:

Human, Rhesus Monkey, Cynomolgus Monkey

UniProt Accession Number of Target Protein: P31358

Alternative Name(s) of Target: CDW52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; YTH66.9HL
Immunogen: Human lymphocytes.

Specificity: Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Antibody first published in: Riechmann L, Clark M, Waldmann H, Winter G. Reshaping human antibodies for therapy. Nature. 1988 Mar 24;332(6162):323-7. PMID:3127726
Note on publication: Describes the reshaping of the rat IgG2b antibody to human IgG1 and shows that it recognises human CD52 antigen.

Immunofluorescence staining of fixed rat thymus tissue with anti-CD52 antibody Campath-1H (Ab00124) Immunofluorescence analysis of fixed rat thymus tissue stained with the chimeric human IgG1 version of Campath-1H (Ab00124-10.0) at 5 µg/ml followed by a FITC conjugated secondary antibody, showing membrane and cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right; Ab00124-10.0 (FITC), DAPI and merged channels.

Immunohistochemical staining of rat epididymis tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat spleen tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Immunohistochemical staining of rat thymus tissue using anti-CD52 antibody (Ab00124) Campath-1H. Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (Ab00124-10.0) was used to stain samples at a concentration of 5 µg/ml.

Flow-cytometry using anti-CD52 antibody Campath-1H (Ab00125) Rhesus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Campath-1H (Ab00125-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD52 antibody Campath-1H (Ab00124) Cynomolgus monkey lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Campath-1H (Ab00728-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescent staining of rat thymus using anti-CD52 antibody Ab00124 Formaldehyde-fixed rat thymus slices were stained with Ab00124-10.0 at 5 µg/ml and detected with a FITC-conjugated secondary antibody. Ab00124 shows cross-reactivity with rat.

Immunohistochemical staining of human thyroid cancer using anti-CD52 antibody Ab00124 Formalin fixed human thyroid cancer slices were were stained with a Ab00124-10.0 at 5 µg/ml.