Human 4-Hydroxynonenal (HNE)ELISA Kit
Cat# CSB-E16214h-96T
Size : 96T
Brand : Cusabio
Contact local distributor :
Phone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Intra-assay Precision (Precision within an assay): CV%<8% | |||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||
Three samples of known concentration were tested in twenty assays to assess. | |||||
To assess the linearity of the assay, samples were spiked with high concentrations of human HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:100 | Average % | 89 | ||||
Range % | 82-96 | |||||
1:200 | Average % | 90 | ||||
Range % | 85-96 | |||||
1:400 | Average % | 85 | ||||
Range % | 81-92 | |||||
1:800 | Average % | 98 | ||||
Range % | 91-104 | |||||
The recovery of human HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 87-101 | ||||
EDTA plasma (n=4) | 98 | 91-104 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ||||||
pg/ml | OD1 | OD2 | Average | |||
2500 | 0.175 | 0.170 | 0.173 | |||
1250 | 0.261 | 0.273 | 0.267 | |||
625 | 0.383 | 0.408 | 0.396 | |||
312.5 | 0.669 | 0.629 | 0.649 | |||
156.25 | 1.008 | 1.021 | 1.014 | |||
78 | 1.345 | 1.353 | 1.349 | |||
39 | 1.879 | 1.784 | 1.832 | |||
0 | 2.611 | 2.525 | 2.568 | |||
and FAQs
This Human HNE ELISA Kit was designed for the quantitative measurement of Human HNE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 19.5 pg/mL.
Citations
- T Zhao, J Afrifa, Y Xue, J Chen, Y Li, D Wang,Free Radical Biology and Medicine,2026
- S Li, M Liu, C Chen, X Li, X Yan, W Zhu,Advanced Science,2026
- C Zhang, DM Su, XQ Wang, P Cao, MD Li, L Fu,Scientific Reports,2025
- Q An, R Wei, Z Huang, Y Tang, M Wang, S He,Acta Pharmaceutica Sinica B,2025
- M Zhao, Y Wu, L Huang, J Wang, A Zhang,Regenerative Therapy,2025
- Insulin Resistance and 4-Hydroxynonenal in Obesity-Associated Lipid Peroxidation of Adipocytes: A Comparative Cross-Sectional Study S Ramsridhar, C Rajkumar, PN Adtani,Journal of International Dental and Medical Research,2024
- Y Huang, R Gan, M Zhang, D Lin, Y Cheng, X Guo,Zygote,2024
- JR Chong, C de Lucia, DA Tovar-Rios,Antioxidants,2024
- A Wu,/,2023
- Z Nie,Cancers,2023
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