HMGB1 ELISA Kit (Rabbit)
Cat# OKWB00408
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
HMGB1 ELISA Kit (Rabbit) (OKWB00408)
| Datasheets/Manuals | Printable datasheet for HMGB1 ELISA Kit (Rabbit) (OKWB00408) |
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| Predicted Species Reactivity | Oryctolagus cuniculus|Rabbit | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Hmgb1 ELISA Kit (Rabbit) (OKWB00408) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hmgb1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hmgb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hmgb1 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625-40ng/ml | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.375 ng/mL | ||||||||||||||||||||||
| Publications | Glycyrrhizic Acid Inhibits High-Mobility Group Box-1 and Homocysteine-Induced Vascular Dysfunction | ||||||||||||||||||||||
| Assay Info | Assay Type: Quantitative Sandwich ELISA |
| Gene Symbol | HMGB1 |
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| Gene Full Name | high mobility group box 1 |
| Alias Symbols | High mobility group protein 1;high mobility group protein B1. |
| NCBI Gene Id | 100328641 |
| Protein Name | High mobility group protein B1|High-mobility group box 1 (Predicted) |
| Uniprot ID | B7NZ89 |
| Protein Accession # | NP_001164752 |
| Nucleotide Accession # | NM_001171281 |
