HigherPurity™ Total RNA Extraction Kit

Cat# AN0280

Size : 50rxn

Brand : Canvax Biotech

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Phone : +1 850 650 7790

HigherPurity™ Total RNA Extraction Kit

Reliable kit for high-yield total RNA purification from multiple sample types.

The HigherPurity™ Total RNA Extraction Kit provides a fast and efficient method for isolating total RNA from a wide range of sources, including animal cells, tissues, bacteria, yeast, fungi, and paraffin-fixed samples.

Using silica spin column chromatography, the kit avoids toxic phenol/chloroform extraction and delivers up to 60 µg of pure RNA in 30–60 minutes. The obtained RNA is free from contaminants and ready for downstream applications such as RT-PCR, qPCR, Northern blotting, and expression analysis.

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SKU: AN0280 Categories: DNA & RNA Extraction, RNA Extraction Kits

Detailed information:

Advantages & Features

  • High yield: up to 60 µg RNA depending on sample type.

  • Safe: no phenol or chloroform required.

  • Fast protocol: 30–60 min workflow.

  • Pure RNA: free of proteins, DNA, and inhibitors.

  • Versatile: compatible with diverse sample types.

Specifications

Specification Description
Sample types Animal cells, tissues, bacteria, yeast, fungi, FFPE
Yield Up to 60 µg total RNA
Technology Silica membrane spin column
Protocol time 30–60 min
Formats 50 rxn, 250 rxn
Includes Buffers, columns, tubes, micropestles
Storage RT (buffers), 4 ºC (RNase-free water)
Intended use Research Use Only

Includes

Includes for 50 rxn:
– 20 mL RNA Buffer Lysis
– 27 mL RNA Wash Buffer 1
– 16 mL RNA Wash Buffer 2
– 5 mL RNase-free ddH2O
– 50 RNAprep spin column
– 50 Filter Column
– 100 Collection tube (2 mL)
– 50 microtube (1.5 mL)
– 50 Micropestle

Download documentation

Applications

  • RT-PCR and qPCR.

  • Northern blotting.

  • Primer extension assays.

  • microRNA cloning and expression arrays.

  • RNA cleanup for transcriptomics.

Tables & Figures

Quality Control

  • Each batch tested for RNA yield, purity (A260/A280 ratio), and integrity.

  • Validated for multiple sample types.

Advice

  • Use micropestles to homogenize tissue samples thoroughly.

  • Always process samples quickly to preserve RNA quality.

Storage, Shipping & Guarantee

  • Shipped in: Ambient Temperature.
  • Storage: Room Temperature (15–25 °C).

Citations

  • Zhou, C., & Liu, S. (2021). Evaluation of the efficacy of MST-312, as a telomerase inhibitor, in the treatment of patients with multiple myeloma after stem cell transplantation. Cellular and Molecular Biology67(4), 115-120.
  • Φορτώση, Αλεξάνδρα Ουρανία. “Συγκριτική μελέτη της μεταβολής της βιολογικής συμπεριφοράς και του φαινοτύπου της καρκινικής κυτταρικής σειράς πνεύμονα Α549, μετά από ανάπτυξη αντοχής στην ακτινοβολία.” (2021).
  • Ortega-Pinazo, J., Serrano-Castro, P. J., Vida-Botella, M., Martínez, B., Pinto-Medel, M. J., Oliver-Martos, B., … & Victoria, U. G. C. (2021). Comparing RNA extraction methods to face the variations in RNA quality using two human biological matrices.
  • Wei, Cen, et al. “LncRNA-IUR Sponges miR-24 to Upregulate P53 in Laryngeal Squamous Cell Carcinoma.” Cancer Management and Research 12 (2020): 11639-11647.
  • Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.
  • Ke, J., Shen, Z., Hu, W., Li, M., Shi, Y., Xie, Z., & Wu, D. (2020). LncRNA DCST1-AS1 Was Upregulated in Endometrial Carcinoma and May Sponge miR-92a-3p to Upregulate Notch1. Cancer Management and Research12, 1221.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

Total RNA extraction kit, RNA isolation kit, RNA purification kit, Total RNA isolation kit, RNA extraction assay

Also known as:

  • Spanish: Kit de extracción de ARN total, Aislamiento de ARN, Purificación de ARN
  • French: Kit d’extraction ARN total, Isolation ARN, Purification ARN
  • German: Gesamt-RNA-Extraktionskit, RNA-Isolation, RNA-Reinigung
  • Italian: Kit estrazione RNA totale, Isolamento RNA, Purificazione RNA

FAQs

Q: What is the maximum yield?
A: Up to 60 µg RNA, depending on sample type.

Q: What types of samples can be processed?
A: Cells, tissues, bacteria, yeast, fungi, and paraffin-fixed samples.

Q: Does it require phenol/chloroform?
A: No, the protocol is phenol-free.

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