H3K36me3 Polyclonal Antibody
Cat# bs-51116R
Size : 50ug
Brand : Bioss
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Histone extracts (15 \u03bcg) from HeLa cells were analysed by Western blot using the H3K36me3 Polyclonal Antibody H3K36me3 (bs-51116R) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk.
A Dot Blot analysis was performed to test the cross reactivity of H3K36me3 Polyclonal Antibody(bs-51116R) with peptides containing other H3 and H4 modifications and unmodified sequence. 0.2-100 pmol of peptide containing histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The result demonstrates high specificity of the antibody for the modification of interest.
Applications
Reactivity
| Overview | |
| Catalog # | bs-51116r-50ug |
| Product Name | H3K36me3 Polyclonal Antibody |
| Applications | WB, ELISA, dot-blot |
| Reactivity | Human, Zebrafish, Drosophila |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Polyclonal antibody raised in rabbit against histone H3, trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide |
| Clonality | Polyclonal |
| Isotype | Not determined |
| Concentration | 0.9ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 8350 |
| Swiss Prot | P68431 |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| dot-blot | |
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