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Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Gpx1 polycolnal antibody (Cat # PAB27563) (0.3ug/ml) staining of rat liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

  • Specifications

    Product Description

    Goat polyclonal antibody raised against synthetic peptide of Gpx1.PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide corresponding to near N-terminus region of mouse Gpx1.

    Sequence

    QSTVYAFSARP

    Host

    Goat

    Theoretical MW (kDa)

    22

    Reactivity

    Chicken, Mouse, Rat

    Purification

    Antigen affinity purification

    Concentration

    0.5 mg/mL

    Recommend Usage

    ELISA (1:64000)
    Western Blot (0.3-1ug/ml)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Gpx1 polycolnal antibody (Cat # PAB27563) (0.3ug/ml) staining of rat liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Gene Info — Gpx1

    Entrez GeneID

    14775

    Protein Accession#

    NP_032186.2

    Gene Name

    Gpx1

    Gene Alias

    AI195024, AL033363, CGPx, GPx-1, GSHPx-1, Gpx

    Gene Description

    glutathione peroxidase 1

    Gene Ontology

    Hyperlink

    Other Designations

    cellular glutathione peroxidase

  • Publication Reference