G6PDX ELISA Kit (Mouse)
Cat# OKEH03202
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
G6PDX ELISA Kit (Mouse) (OKEH03202)
| Datasheets/Manuals | Printable datasheet for G6PDX ELISA Kit (Mouse) (OKEH03202) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology G6PDX ELISA Kit (Mouse) (OKEH03202) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for G6pdx has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for G6pdx is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample G6pdx captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.78-50mIU/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 6.7% (n = 20) Mean Inter-assay CV%: <= 8.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.39mIU/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Glucose-6-phosphate 1-dehydrogenase X | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | G6pdx |
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| Gene Full Name | glucose-6-phosphate dehydrogenase X-linked |
| Alias Symbols | G28, G28A, G6p, G6pd, glucose-6-phosphate 1-dehydrogenase X, Gpd, Gpdx. |
| NCBI Gene Id | 14381 |
| Protein Name | Glucose-6-phosphate 1-dehydrogenase X |
| Description of Target | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity). |
| Uniprot ID | Q00612 |
| Protein Accession # | NP_032088.1 |
| Nucleotide Accession # | NM_008062.2 |
| Protein Size (# AA) | 515 |
| Molecular Weight | 59 kDa |
