G418 Sulfate is an aminoglycoside related to Gentamycin, used as a selective agent in bacterial genetics. It is toxic to yeast, bacteria, many plant and mammalian cells, helminths and protozoans.

Synonyms:
O-2-Amino-2,7-dideoxy-D-glycero-α-D-gluco-heptopyranosyl-(1→4)-O-[3-deoxy-4-C-methyl-3-(methylamino)-β-L-arabinopyranosyl-(1→6)]-2-deoxy-D-streptamine Sulfate (1:2); Antibiotic G 418 Sulfate; G418 Sulfate

CAS No:
108321-42-2

Molecular Formula:
C20H40N4O10•2H2SO4

Molecular Weight:
692.71

Appearance:
White, crystalline powder

Solubility:
Colorless, clear, complete

Potency:
≥620ug/mg

Absorbance (1mg/ml):
280nm: ≤0.015
570nm: ≤0.10

Water (KF):
≤12%

Specific Rotation:
+104° to +121ºC

Suggested Working Concentrations:
100-800ug/ml
Mammalian Selection: 400ug/ml
Maintenance: 200ug/ml

Reconstitution:
Reconstitute with water or ethanol to a desired stock concentration. Sterilize by filtration through a 0.2um filter, aliquot and freeze at -20°C.

Storage and Stability:
Powder: Store at 4ºC. Avoid moisture uptake, as this can decrease potency. Stable for 12 months after receipt.
Stock solution: Aliquot and store at -20°C. Stable for 3-6 months -at 20°C.