Western Blot (Cell lysate)
FOS polycolnal antibody (Cat # PAB27545) (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of FOS.
Immunogen
A synthetic peptide corresponding to amino acids 283-295 at internal region of human FOS.
Sequence
SETARSVPDMDLS
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Cat, Chicken, Dog, Human, Pig
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.3-1ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
FOS polycolnal antibody (Cat # PAB27545) (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. - Gene Info — FOS
Entrez GeneID
2353Protein Accession#
NP_005243.1Gene Name
FOS
Gene Alias
AP-1, C-FOS
Gene Description
v-fos FBJ murine osteosarcoma viral oncogene homolog
Omim ID
164810Gene Ontology
HyperlinkGene Summary
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq
Other Designations
FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|activator protein 1|cellular oncogene c-fos
- Interactomes
- Pathways
- Diseases