Fluid Thioglycollate Medium w/Beef Extract (Powder)

Fluid Thioglycollate Medium with 0.5% Beef Extract is recommended by Animal and Plant Health Inspection Service, USDA, in the detection of viable bacteria in live vaccines. Various workers described the use of sulfhydryl compounds and low concentrations of agar, alone or in combination, to culture anaerobic organisms in a fluid broth under aerobic conditions. Other workers described the use of sodium thioglycollate in these broth media for the neutralization of mercurial preservatives. Sodium thioglycollate aIso lowers the oxidation-reduction potential of the media. Resazurin indicates the status of oxidation or aerobiosis. Dextrose is added to enhance growth.

Appearance:
Light beige, homogeneous, free flowing powder.

pH (25°C):
7.2±0.2

Solubility:
Yellow, hazy to clear, complete with upper 10% of solution light to medium red upon standing.

Microbiological Analysis:
Bacillus subtilis ATCC 9372: Growth
Bacteroides vulgatus ATCC 8482: Growth
Candida albicans ATCC 10231: Growth
Clostridium sporogenes ATCC 11437: Growth
Micrococcus luteus ATCC 9341: Growth

Recommended by Agriculture Research Service CFR Amendment 70-253, 35FR16038, 10, 13, 70,

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige.