Fish dopamine(DA) ELISA kit
Cat# CSB-EQ027496FI-24T
Size : 24T
Brand : Cusabio
Contact local distributor :
Phone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
dopamine(DA)
Abbreviation
DA
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
62.5 pg/mL-1000 pg/mL
Sensitivity
62.5 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
Recovery
| The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish dopamine (DA) ELISA Kit was designed for the quantitative measurement of Fish dopamine (DA) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 62.5 pg/mL.
Citations
- Developmental neurotoxic effects of dibutyl phthalate and its metabolite mono-n-butyl phthalate on Danio rerio S Lee, S Lee, K Eghan, WK Kim,Ecotoxicology and Environmental Safety,2026
- S Ferrara, P Mikula, A Hollerova, P Marsalek, F Tichy,Applied Sciences,2025
- PG Kraus, YJ Choi, LS Lee,Environmental Science & Technology,2025
- 조아현,Thesis & Dissertation,2025
- SC Stradtman, JN Swihart, K Moore, IN Akoro,International Journal of Molecular Sciences,2024
- S Biswas,Journal of Ayurveda and integrative medicine,2023
- O Wasel,Environmental science & technology,2023
- K Yun,Environmental pollution,2023
- Y Tang,Aquatic toxicology,2023
- CH Lee,/,2024
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