ECL Western Blotting Substrate is a sensitive, nonradioactive, and upgraded luminol-based chemiluminescent substrate for easy detection of HRP (horseradish peroxidase) on immunoblots. The substrate provides excellent signal and clear background. Blots can be exposed to X-ray film or a CCD camera in multiple time to receive optimal results or stripped of the immunodetection reagents and re-probed. This ECL Western Blotting substrate is an optimal substitute for Amersham ECL substrate and no extra steps for assay optimization are needed.