Size : 50rxns
Phone : +1 850 650 7790
This kit employs an enzymatic method to lyse cells and utilizes a silica membrane spin column to specifically bind to DNA. It is suitable for efficiently extracting genomic DNA from various materials (animal cells, animal tissues, mouse tails, E. coli, yeast). The extracted DNA is applicable for experiments such as enzymatic digestion, PCR, and Southern Blot.
Features
• Fast extraction, high yield (up to 15 μg).
• High integrity of DNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.
• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.
at 15℃-30℃ in a dry place for two years.
At room temperature.
Research Use Only
Component | EE101-01/-11 (50 rxns) | EE101-02/-12 (200 rxns) |
| Lysis Buffer 2 (LB2) | 6 ml | 24 ml |
| Binding Buffer 2 (BB2) | 28 ml | 110 ml |
| Clean Buffer 2 (CB2) | 15 ml | 60 ml |
| Wash Buffer 2 (WB2) | 12 ml | 2×22 ml |
| Elution Buffer (EB) | 25 ml | 80 ml |
| RNase A (20 mg/ml) | 1 ml/- | 4×1 ml /- |
| Proteinase K (20 mg/ml) | 1 ml | 4×1 ml |
| Genomic Spin Columns with Collection Tubes | 50 each | 200 each |
1 Liu S, Zhang M, Bao Y, et al. Characterization of a Highly Selective 2 ″-O-Galactosyltransferase from Trollius chinensis and structure-guided engineering for improving UDP-glucose selectivity[J]. Organic Letters, 2021.(IF 6.00)
2 Li W, Ali T, Zheng C, et al. Anti-depressive-like behaviors of APN KO mice involve Trkb/BDNF signaling related neuroinflammatory changes[J]. Molecular Psychiatry, 2022.(IF 15.99)
3 Wei G, Sun H, Dong K, et al. The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy[J]. Cell metabolism, 2021.(IF 27.28)
4 Song X, Yang F, Yang T, et al. Phase separation of EB1 guides microtubule plus-end dynamics[J]. Nature Cell Biology,2023.(IF 28.21)
5 She X, Shen S, Chen G, et al. Immune surveillance of brain metastatic cancer cells is mediated by IFITM1[J]. The EMBO Journal, 2023.(IF 14.01)
Manual