D-(+)-Melezitose hydrate [207511-10-2]

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Cat# NB-64-19093-500mg

Size : 500mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

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Contact local distributor :

Phone : +1 850 650 7790

D-(+)-Melezitose hydrate (Synonyms: melicitose hydrate, Melezitose hydrate, D-Melezitose hydrate)

Catalog No. T3016 Copy Product Info

Purity: 99.45%

D-(+)-Melezitose hydrate (melicitose) is a nonreducing trisaccharide sugar that is produced by many plant sap-eating insects, including aphids such as Cinara pilicornis by an enzyme reaction.

D-(+)-Melezitose hydrate

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Synonyms melicitose hydrate, Melezitose hydrate, D-Melezitose hydrate

D-(+)-Melezitose hydrate (melicitose) is a nonreducing trisaccharide sugar that is produced by many plant sap-eating insects, including aphids such as Cinara pilicornis by an enzyme reaction.

Cas No. 207511-10-2

Batch Information

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Purity:99.45%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	D-(+)-Melezitose hydrate (melicitose) is a nonreducing trisaccharide sugar that is produced by many plant sap-eating insects, including aphids such as Cinara pilicornis by an enzyme reaction.
Kinase Assay	FlashPlate assay (96-well screening assay): To columns 1 through 10, 1 μL of Olaparib (in DMSO) is added, and 1 μL DMSO only is added to the positive (POS) and negative (NEG) control wells (columns 11 and 12, respectively) of a pretreated FlashPlate. PARP-1 is diluted 1:40 in buffer (buffer B: 10% glycerol (v/v), 25 mM HEPES, 12.5 mM MgCl2,50 mM KCl, 1 mM DTT, 0.01% NP-40 (v/v), pH 7.6) and 40 μL added to all 96 wells (final PARP-1 concentration in the assay is ~1 ng/μL). The plate is sealed and shaken at RT for 15 min. Following this, 10 μL of positive reaction mix (0.2 ng/μL of double-stranded oligonucleotide [M3/M4] DNA per well, 5 μM of NAD+ final assay concentration, and 0.075 μCi 3H-NAD+ per well) is added to the appropriate wells (columns 1-11). The negative reaction mix, lacking the DNA oligonucleotide, is added to column 12 (with the mean negative control value used as the background). The plate is resealed and shaken for a further 60 min at RT to allow the reaction to continue. Then, 50 μL of ice-cold acetic acid (30%) is added to each well to stop the reaction, and the plate is sealed and shaken for a further 60 min at RT. Tritiated signal bound to the FlashPlate is then determined in counts per minute (CPM) using the TopCount plate reader.
Synonyms	melicitose hydrate, Melezitose hydrate, D-Melezitose hydrate

Chemical Properties

Molecular Weight	522.45
Formula	C₁₈H₃₄O₁₇
Cas No.	207511-10-2
Smiles	O.OC[C@H]1O[C@@](CO)(O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H]1O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (114.84 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9141 mL	9.5703 mL	19.1406 mL	95.7029 mL
5 mM	0.3828 mL	1.9141 mL	3.8281 mL	19.1406 mL
10 mM	0.1914 mL	0.9570 mL	1.9141 mL	9.5703 mL
20 mM	0.0957 mL	0.4785 mL	0.9570 mL	4.7851 mL
50 mM	0.0383 mL	0.1914 mL	0.3828 mL	1.9141 mL
100 mM	0.0191 mL	0.0957 mL	0.1914 mL	0.9570 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.