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Colchicine [64-86-8]

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Cat# T0320-1mL

Size : 1mL

Brand : TargetMol

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Colchicine

Catalog No. T0320   CAS 64-86-8
Synonyms: Colcin, Colchineos, Colchisol

Colchicine (Colcin) is a natural product that is an inhibitor of microtubule polymerization (IC50=3 nM) and blocks microtubule polymerization by binding to microtubule proteins. Colchicine can be used in the treatment of ventilation and rheumatic diseases.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Colchicine Chemical Structure
Colchicine, CAS 64-86-8
Description Colchicine (Colcin) is a natural product that is an inhibitor of microtubule polymerization (IC50=3 nM) and blocks microtubule polymerization by binding to microtubule proteins. Colchicine can be used in the treatment of ventilation and rheumatic diseases.
Targets&IC50 Microtubule:3 nM
In vitro METHODS: Human pharyngeal carcinoma cells FaDu and SNU1041 were treated with Colchicine (0.0-1 μM) for 24-72 h. Cell viability was measured by XTT assay.
RESULTS: Colchicine treatment was cytotoxic to both FaDu and SNU1041 cell lines in a dose- and time-dependent manner. [1]
METHODS: Chorionic villous cells AFCs and amniotic fluid cells CVCs were treated with Colchicine (0.15 μg/mL) for 3-24 h. Apoptosis was detected by Flow Cytometry.
RESULTS: Colchicine induced a significant increase in the proportion of annexin V and PI double positive cells. [2]
In vivo METHODS: To investigate the antitumor activity, Colchicine (0.1 mg/kg) was orally administered to BALB/c-nu mice bearing the human pharyngeal cancer tumor FaDu every two days for fourteen days.
RESULTS: Colchicine was effective in inhibiting tumor growth in a hypopharyngeal cancer model nude mouse without serious complications. [1]
METHODS: To investigate the effect of anti-Fas antibody-induced lethality, Colchicine (2 mg/kg) was injected intraperitoneally into C57BL/6 mice, followed by Jo2 antibody (10 μg) 24 h later.
RESULTS: All mice treated with Colchicine survived the lethal attack.Colchicine reduced the susceptibility of mice to the lethal effect of Jo2 against Fas antibody. [3]
Animal Research a C57BL/6 background are used. To examine the effects of Colchicine on NSAID-induced small intestinal injury, vehicle or Colchicine (1 or 3 mg/kg) is administered orally 30 min prior to indomethacin administration. Mice received intraperitoneal injections of sterilized phosphate buffered saline or mouse recombinant IL-1β (0.1 μg/kg) 3 h after indomethacin treatment. Vehicle or Colchicine (1 or 3 mg/kg) is also administered to NLRP3?/? mice before indomethacin administration. The lesion index is evaluated 24 h after indomethacin administration and examined mRNA and protein expression of inflammasome components 6 h after indomethacin administration.
Source
Synonyms Colcin, Colchineos, Colchisol
Molecular Weight 399.44
Formula C22H25NO6
CAS No. 64-86-8

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 39.9 mg/mL(100 mM)

H2O: 1.33 mg/mL(3.34 mM), Sonification is recommended

TargetMolReferences and Literature

1. Cho JH, et al. Anticancer Effects of Colchicine on Hypopharyngeal Cancer. Anticancer Res. 2017 Nov;37(11):6269-6280. 2. Wang D, et al. Colchicine causes prenatal cell toxicity and increases tetraploid risk. BMC Pharmacol Toxicol. 2019 Nov 13;20(1):66. 3. Feng G, et al. Colchicine protects mice from the lethal effect of an agonistic anti-Fas antibody. J Clin Invest. 2000 Feb;105(3):329-39. 4. Lin Y, Luo T, Weng A, et al. Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling[J]. Frontiers in Immunology. 2020, 11: 3197. 5. Dong H, Sun H, Zheng J. A microchip for integrated single-cell genotoxicity assay[J]. Talanta. 2016, 161: 804-811. 7. Zeng X, Zhu S, Lu W, et al. Target identification among known drugs by deep learning from heterogeneous networks[J]. Chemical Science. 2020, 11(7): 1775-1797.

TargetMolCitations

1. Wang D, Wang Y, Di X, et al.Cortical tension drug screen links mitotic spindle integrity to Rho pathway.Current Biology.2023 2. Wang H, Liang Y, Zhao L, et al.miR-653-3p promotes genomic instability of colorectal cancer cells via targeting SIRT1/TWIST1 signaling pathway.Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease.2023: 166821. 3. Yuan W, Liu T, Wang Y, et al.Autophagy induced by PP121 alleviates MSU crystal-induced acute gouty arthritis via inhibition of the NLRP3 inflammasome.International Immunopharmacology.2023, 123: 110756. 4. Hou Z, Lin S, Du T, et al.S-72, a Novel Orally Available Tubulin Inhibitor, Overcomes Paclitaxel Resistance via Inactivation of the STING Pathway in Breast Cancer.Pharmaceuticals.2023, 16(5): 749. 5. Dong H, Sun H, Zheng J. A microchip for integrated single-cell genotoxicity assay. Talanta. 2016, 161: 804-811. 6. Pan H, Lin Y, Dou J, et al. Wedelolactone facilitates Ser/Thr phosphorylation of NLRP3 dependent on PKA signalling to block inflammasome activation and pyroptosis. Cell Proliferation. 2020, 53(9): e12868 7. Ai, Yongqiang, et al. The Combination of Schisandrol B and Wedelolactone Synergistically Reverses Hepatic Fibrosis Via Modulating Multiple Signaling Pathways in Mice. Frontiers in Pharmacology. 12 (2021) 8. Lin Y, Luo T, Weng A, et al. Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling. Frontiers in Immunology. 2020 Dec 7;11:580593. doi: 10.3389 9. Zeng X, Zhu S, Lu W, et al. Target identification among known drugs by deep learning from heterogeneous networks. Chemical Science. 2020, 11(7): 1775-1797. 10. Li X, Liu B, Wen Y, et al.Coordination of RAB-8 and RAB-11 during unconventional protein secretion.Journal of Cell Biology.2023, 223(2): e202306107.