Reagents and instruments for immunology, cell biology and molecular biology.
 
> Cetrimonium (bromide) [57-09-0]

Cetrimonium (bromide) [57-09-0]

Print Print
Please log in

Cat# HY-B1260-100mg

Size : 100mg

Brand : MedChemExpress

Contact local distributor :


Phone : +1 850 650 7790

Description

Cetrimonium bromide (CTAB), a quaternary ammonium, is an orally active cationic surfaetant. Cetrimonium bromide has toxicity and anticancer effect. Cetrimonium bromide inhibits cell migration and invasion through modulating the canonical and non-canonical TGF-β signaling pathways. Cetrimonium bromide can be used for DNA extraction[1][2][3][4].

IC50 & Target

MMP-9

 

MMP-2

 

In Vitro

Cetrimonium bromide (1-5 μM, 24 h) cannot affect growth of SK-HEP-1 cells[1].
Cetrimonium bromide (5 μM, 16-24 h) attenuates cellular migration and invasion of SK-HEP-1cells[1].
Cetrimonium bromide (5 μM, 24 h) inhibits the protein expression of MMP-2 and MMP-9, increases the protein expression of TIMP-1 and TIMP-2 and restrain the protein expression of Rac1, cdc42 and RhoA in SK-HEP-1cells[1].
Cetrimonium bromide (5 μM, 12-72 h) induces apoptosis in human Head and neck cancer (HNC) cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cetrimonium bromide Related Antibodies

Cell Viability Assay[1]

Cell Line: SK-HEP-1 cells
Concentration: 1 μM, 2.5 μM, 5 μM
Incubation Time: 24 h
Result: Retained more than 90% cell viability at 24 hours.

Cell Cycle Analysis[2]

Cell Line: HNC cells
Concentration: 5 μM
Incubation Time: 12 h, 24 h, 48 h, 72 h
Result: Induced caspase activation.
Observed the activation of the caspase cascade and increased in a time-dependent manner.

Western Blot Analysis[1]

Cell Line: SK-HEP-1 cells
Concentration: 1 μM, 2.5 μM, 5 μM
Incubation Time: 24 h
Result: Significantly down-regulates MMP-2 and MMP-9 expressions in SK-HEP-1 cells in a dose-dependent manner.
Significantly increased the protein expression of TIMP-1 and TIMP-2.
Negatively affects the protein levels of Rac1, cdc42 and RhoA in SK-HEP-1 cells.
In Vivo

Cetrimonium bromide (5 mg/kg, Intraperitoneal injection, once a day for five consecutive days) ablated tumor-forming capacity of FaDu cells and delayed growth of established tumors in FaDu cells tumor-bearing mice[2].
Cetrimonium bromide (10-45 mg/kg, Supplemented in drinking water, once a day for 1 year) has subacute and chronic toxicity in the rat[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: FaDu cells tumor-bearing mice[2]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Induced a modest reduction in tumor development.
Delayed the mean time to reach a tumor-plus-leg diameters (TLD) of 14mm by ~3.7 days.
Animal Model: Sprague-Dawley rats[3]
Dosage: 10 mg/kg, 20 mg/kg, 45 mg/kg
Administration: Supplemented in drinking water
Result: Showed lower body weight at highest concentration within 3 weeks.
Reduced the body weight throughout the experimental in males and skeletal growth at highest dose level in both males and females.
Significantly decreased efficiency of food conversion in male rats receiving the highest dose.
Molecular Weight

364.45

Formula

C19H42BrN
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCC[N+](C)(C)C.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (27.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4.55 mg/mL (12.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7439 mL 13.7193 mL 27.4386 mL
5 mM 0.5488 mL 2.7439 mL 5.4877 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1 mg/mL (2.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (137.19 mM); Clear solution; Need ultrasonic
Purity & Documentation
References

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT